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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB10080-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IFN-alpha I Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IFN-alpha I in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01571- Isotype
- IgG
- Antibody clone number
- 995826
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IFN-alpha I Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN-alpha I Antibody. Recombinant Human IFN-alpha I reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-alpha I (20 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IFN-alpha I Monoclonal Antibody (Catalog # MAB10080). The ND50 is typically 0.6-6 ng/mL.
