Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00065008-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00065008-M02, RRID:AB_1577419
- Product name
- MRPL1 monoclonal antibody (M02), clone 2C4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant MRPL1.
- Antigen sequence
MVYQTSLCSCSVNIRVPNRHFAAAKKSAKKTKKGA
KEKTPDEKKDEIEKIKAYPYMEGEPEDDVYLKRLY
PRQIYEVEKAVHLLKKFQILDFTSPKQSVYLDLTL
DMALGKKKNVEPFTSVLSLPYPFASEINKVAVFTE
NASEVKIAEENGAASAGGTSLIQKIWDDEIVADFY
VAVPEIMPELNRLRKKLNKKYPKLSRNSIGRDIPK
MLELFKNGHEIKVDEERENFLQTKIATLDMSSDQI
AANLQAVINEVCRHRPLNLGPFVVRAFLRSSTSEG
LLLKIDPLLPKEVKNEESEKEDA- Isotype
- IgG
- Antibody clone number
- 2C4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MRPL1 monoclonal antibody (M02), clone 2C4. Western Blot analysis of MRPL1 expression in A-431(Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MRPL1 expression in transfected 293T cell line by MRPL1 monoclonal antibody (M02), clone 2C4.Lane 1: MRPL1 transfected lysate(34.5 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MRPL1 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MRPL1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol