Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-29000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADAM15 Recombinant Rabbit Monoclonal Antibody (7)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
- Antibody clone number
- 7
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of Human ADAM15 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000, 1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed, paraffin-embedded human heart showing blood vessel staining (red arrow) using ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000, 1:200).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of formalin fixed, paraffin-embedded human lymph node showing membrane staining of endothelial cells (red arrow) using ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000, 1:2,000).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Profile of anti-ADAM15 reactivity on MCF-7 cells analyzed by flow cytometry. Cells were stained with an ADAM15 Monoclonal Antibody (Product # MA5-29000)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Profile of ADAM15 on MCF-7 cells analyzed by flow cytometry using ADAM15 Recombinant Rabbit Monoclonal Antibody (7) (Product # MA5-29000).