Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB600-276 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-276, RRID:AB_2281235
- Product name
- Rabbit Polyclonal LEO1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Differential usage of alternate promoters of the human stress response gene ATF3 in stress response and cancer cells.
Miyazaki K, Inoue S, Yamada K, Watanabe M, Liu Q, Watanabe T, Adachi MT, Tanaka Y, Kitajima S
Nucleic acids research 2009 Apr;37(5):1438-51
Nucleic acids research 2009 Apr;37(5):1438-51
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEO1 Antibody [NB600-276] - Detection of Human Leo1on HeLa whole cell lysate using NB600-276. In B, Leo1 was immunoprecipitated by using NB600-276 at 3 mcg/10mg with or without blocking peptide.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, MCF-7, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody [NB600-276] used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by western blot. Samples: Whole cell lysate (15 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Leo1 antibody [NB600-276] used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LEO1 Antibody [NB600-276] - Detection of human Leo1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Leo1 antibody [NB600-276] (lot 3) used for IP at 6 ug per reaction. Leo1 was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated Leo1, NB600-276 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: LEO1 Antibody [NB600-276] - Sample: FFPE section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-LEO1 used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: LEO1 Antibody [NB600-276] - Detection of mouse Leo1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Leo1 (NB600-276). Detection: DAB