PA5-118062

antibody from Invitrogen Antibodies

Targeting: MID1IP1 FLJ10386, G12-like, MIG12, STRAIT11499, THRSPL

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118062

Provider

Invitrogen Antibodies

Product name

MID1IP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MID1IP1 Polyclonal Antibody (Product # PA5-118062) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: HEK293 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 25 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MID1IP1 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with MID1IP1 Polyclonal Antibody (Product # PA5-118062) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: MID1IP1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa. Observed band size: 25 kDa.