H00002712-M04

antibody from Abnova Corporation

Targeting: GK2 GKP2, GKTA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002712-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002712-M04, RRID:AB_1204403

Product name

GK2 monoclonal antibody (M04), clone 3G4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant GK2.

Antigen sequence

MAAPKTAAVGPLVGAVVQGTNSTRFLVFNSKTAEL
LSHHKVELTQEFPKEGWVEQDPKEILQSVYECIAR
TCEKLDELNIDISNIKAVGVSNQRETTVIWDKLTG
EPLYNAVVWLDLRTQTTVEDLSKKIPGNSNFVKSK
TGLPLSTYFSAVKLRWMLDNVRNVQKAVEEGRALF
GTIDSWLIWSLTGGVNGGVHCTDVTNASRTMLFNI
HSLEWDKELCDFFEIPMDLLPNVFSSSEIYGLIKT
GALEGVPISGCLGDQCAALVGQMCFQEGQAKNTYG
TGCFLLCNTGRKCVFSEHGLLTTVAYKLGREKPAY
YALEGSVAIAGAVIRWLRDNLGIIETSGDIERLAK
EVGTSYGCYFVPAFSGLYAPYWEPSARGILCGLTQ
FTNKCHIAFAALEAVCFQTREILEAMNRDCGIPLR
HLQVDGGMTNNKVLMQLQADILHIPVIKPFMPETT
ALGAAMAAGAAEGVSVWSLEPQALSVLRMERFEPQ
IQATESEIRYATWKKAVMKSMGWVTSQSPEGGDPS
IFSSLPLGFFIVSSMVMLIGARYISGVP


Isotype

IgG

Antibody clone number

3G4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of GK2 expression in transfected 293T cell line by GK2 monoclonal antibody (M04), clone 3G4.Lane 1: GK2 transfected lysate(60.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged GK2 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol