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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP53589PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP53589PU-N, RRID:AB_11145622
- Product name
- anti RASGEF1B (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the central region (between 131-160aa) of human RASGEF1B.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in MCF-7 cell line lysates (35ug/lane) using RASGEF1B antibody Cat.-No. AP53589PU-N. This demonstrates the RASGEF1B antibody detected the RASGEF1B protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in mouse cerebellum tissue lysates (35ug/lane) using RASGEF1B antibody Cat.-No. AP53589PU-N. This demonstrates the RASGEF1B antibody detected the RASGEF1B protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human esophageal carcinoma (Formalin-fixed, Paraffin-embedded) using RASGEF1B antibody Cat.-No. AP53589PU-N, followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of the RASGEF1B antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram) using RASGEF1B antibody Cat.-No. AP53589PU-N, followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
