Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [15]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-89480 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89480, RRID:AB_11021912
- Product name
- Rabbit Polyclonal PPHLN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human PPHLN1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells.
Tchasovnikarova IA, Timms RT, Matheson NJ, Wals K, Antrobus R, Göttgens B, Dougan G, Dawson MA, Lehner PJ
Science (New York, N.Y.) 2015 Jun 26;348(6242):1481-1485
Science (New York, N.Y.) 2015 Jun 26;348(6242):1481-1485
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPHLN1 Antibody [NBP1-89480] - Analysis in human cell line HELA.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPHLN1 Antibody [NBP1-89480] - Analysis using Anti-PPHLN1 antibody NBP1-89480 (A) shows similar pattern to independent antibody NBP2-38431 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PPHLN1 Antibody [NBP1-89480] - Human foreskin fibroblasts whole cell lysates. Antibody at 1:1000. Image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human pancreas shows no positivity in exocrine glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human skin shows weak nuclear positivity in keratinocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human lymph node shows moderate nuclear positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Immunohistochemistry analysis in human testis and pancreas tissues. Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human liver.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human cerebral cortex, colon, liver and testis using Anti-MAPRE2 antibody (A) NBP1-89480 shows similar protein distribution across tissues to independent antibody NBP2-38431 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Analysis in human testis and pancreas tissues. Corresponding PPHLN1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human pancreas shows negative to very weak positivity in exocrine glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human liver shows weak nuclear positivity in hepatocytes.