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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010210-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010210-M01, RRID:AB_548770
- Product name
- Mouse Monoclonal TOPORS Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. TOPORS - topoisomerase I binding, arginine/serine-rich
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Plk1 phosphorylation of Topors is involved in its degradation.
Mitotic phosphorylation stimulates DNA relaxation activity of human topoisomerase I.
Yang X, Li H, Deng A, Liu X
Molecular biology reports 2010 Jul;37(6):3023-8
Molecular biology reports 2010 Jul;37(6):3023-8
Mitotic phosphorylation stimulates DNA relaxation activity of human topoisomerase I.
Hackbarth JS, Galvez-Peralta M, Dai NT, Loegering DA, Peterson KL, Meng XW, Karnitz LM, Kaufmann SH
The Journal of biological chemistry 2008 Jun 13;283(24):16711-22
The Journal of biological chemistry 2008 Jun 13;283(24):16711-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TOPORS Antibody (5G11) [H00010210-M01] - TOPORS expression in HeLa NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: TOPORS Antibody (5G11) [H00010210-M01] - Detection limit for recombinant GST tagged TOPORS is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TOPORS Antibody (5G11) [H00010210-M01] - Analysis of monoclonal antibody to TOPORS on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TOPORS Antibody (5G11) [H00010210-M01] - Analysis of monoclonal antibody to TOPORS on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. Antibody concentration 3 ug/ml.
