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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-19829 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- For Western Blot, this antibody has non-specific bands at 34 kDa and 92 kDa.
- Concentration
- 0.9 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TE 671 Whole Cell Lysate using Product # PA5-19829, BRD1 primary antibody at a dilution of 1 µg/mL (lane 1). Staining of Raji Whole Cell Lysate at a dilution of 1 µg/mL (lane 2). Blot treated with a secondary HRP-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:3000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MCF7 cells using Product # PA5-19829, anti-BRD1 antibody. The cells were fixed with methanol (100%) for 5 minutes, permabilised with BSA (1%), normal goat serum (10%) and glycine (0.3 M) in 0.1% T-BST for 1 hour and exposed to the primary antibody at a concentration of 1 µg/mL overnight at 4C. The secondary antibody was a 448 fluorescence conjugated Goat anti-rabbit IgG (green) at a dilution of 1:1000. A WGA- 594 fluorescent conjugated stain was used to label plasma membranes (red) and the nuclei stain was DAPI (blue).
