Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-60411 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60411, RRID:AB_905311
- Product name
- Rabbit Polyclonal CHD1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A regulatory role for CHD2 in myelopoiesis.
Integrating phosphoproteomics into the clinical management of prostate cancer.
Recurrent deletion of CHD1 in prostate cancer with relevance to cell invasiveness.
Identification of novel CHD1-associated collaborative alterations of genomic structure and functional assessment of CHD1 in prostate cancer.
Treatment of breast cancer cells with DNA demethylating agents leads to a release of Pol II stalling at genes with DNA-hypermethylated regions upstream of TSS.
Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.
Shahin Varnoosfaderani F, Palau A, Dong W, Persson J, Durand-Dubief M, Svensson JP, Lennartsson A
Epigenetics 2020 Jun-Jul;15(6-7):702-714
Epigenetics 2020 Jun-Jul;15(6-7):702-714
Integrating phosphoproteomics into the clinical management of prostate cancer.
Cheng LC, Tan VM, Ganesan S, Drake JM
Clinical and translational medicine 2017 Dec;6(1):9
Clinical and translational medicine 2017 Dec;6(1):9
Recurrent deletion of CHD1 in prostate cancer with relevance to cell invasiveness.
Huang S, Gulzar ZG, Salari K, Lapointe J, Brooks JD, Pollack JR
Oncogene 2012 Sep 13;31(37):4164-70
Oncogene 2012 Sep 13;31(37):4164-70
Identification of novel CHD1-associated collaborative alterations of genomic structure and functional assessment of CHD1 in prostate cancer.
Liu W, Lindberg J, Sui G, Luo J, Egevad L, Li T, Xie C, Wan M, Kim ST, Wang Z, Turner AR, Zhang Z, Feng J, Yan Y, Sun J, Bova GS, Ewing CM, Yan G, Gielzak M, Cramer SD, Vessella RL, Zheng SL, Grönberg H, Isaacs WB, Xu J
Oncogene 2012 Aug 30;31(35):3939-48
Oncogene 2012 Aug 30;31(35):3939-48
Treatment of breast cancer cells with DNA demethylating agents leads to a release of Pol II stalling at genes with DNA-hypermethylated regions upstream of TSS.
Tao Y, Liu S, Briones V, Geiman TM, Muegge K
Nucleic acids research 2011 Dec;39(22):9508-20
Nucleic acids research 2011 Dec;39(22):9508-20
Lsh mediated RNA polymerase II stalling at HoxC6 and HoxC8 involves DNA methylation.
Tao Y, Xi S, Briones V, Muegge K
PloS one 2010 Feb 11;5(2):e9163
PloS one 2010 Feb 11;5(2):e9163
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CHD1 Antibody [NB100-60411] - Detection of Human CHD1 on HeLa whole cell lysate using NB100-60411. CHD1 was also immunoprecipitated by rabbit anti-CHD1 antibody NB100-60410.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: CHD1 Antibody [NB100-60411] - ChIP-chip scatter plot of anti-CHD1 (NB100-60411) enriched DNA binding sites versus input reference DNA. CHD1 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-CHD1 ChIP, normal rabbit IgG showed little enrichment.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation Sequencing: CHD1 Antibody [NB100-60411] - Localization of CHD1 Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-CHD1 antibody NB100-60411 and analyzed by DNA sequencing. The figure illustrates the peak distribution of CHD1 binding within a 3.5 Mb region of the human X chromosome as detected using anti-CHD1 antibody NB100-60411. ChIP-seq validation performed by Diogenode, Denville, NJ.