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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-16746 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STEP Monoclonal Antibody (23E5)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Suggested positive control: rat brain.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 23E5
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5.
Brigidi GS, Santyr B, Shimell J, Jovellar B, Bamji SX
Nature communications 2015 Sep 3;6:8200
Nature communications 2015 Sep 3;6:8200
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of STEP protein in striatal rat protein homogenates, using Product # MA1-16746.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STEP in striatal rat protein homogenates. Sample was incubated in STEP monoclonal antibody (Product # MA1-16746).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of STEP in immersion fixed paraffin-embedded sections of human brain basal ganglia. Samples were incubated in STEP monoclonal antibody (Product # MA1-16746) using a dilution of 1:100 for 1 hour at room temperature followed by the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 PSD-95 enhances the binding and tyrosine phosphorylation of DHHC5 by Fyn kinase. ( a - d ) Fourteen DIV hippocampal neurons were stimulated, lysed at the indicated time points, immunoprecipitated with anti- ( a ) DHHC5 or ( c ) Fyn and blots probed with the indicated antibodies. ( a , b ) DHHC5 tyrosine phosphorylation (phY; P =0.007, F 6,14 =7.749) and DHHC5/Fyn interactions ( P
