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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [34]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB300-202 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-202, RRID:AB_10107652
- Product name
- Mouse Monoclonal STEP Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. NB300-202 is specific for STEP61, STEP46, STEP38, and STEP20.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chronic Stress Induces Maladaptive Behaviors by Activating Corticotropin-Releasing Hormone Signaling in the Mouse Oval Bed Nucleus of the Stria Terminalis.
Selective reduction of APP-BACE1 activity improves memory via NMDA-NR2B receptor-mediated mechanisms in aged PDAPP mice.
Age-related NMDA signaling alterations in SOD2 deficient mice.
Excitotoxic inactivation of constitutive oxidative stress detoxification pathway in neurons can be rescued by PKD1.
Aging is associated with dimerization and inactivation of the brain-enriched tyrosine phosphatase STEP.
Zn2+-dependent Activation of the Trk Signaling Pathway Induces Phosphorylation of the Brain-enriched Tyrosine Phosphatase STEP: MOLECULAR BASIS FOR ZN2+-INDUCED ERK MAPK ACTIVATION.
PSD-95 stabilizes NMDA receptors by inducing the degradation of STEP61.
A single fraction from Uncaria sinensis exerts neuroprotective effects against glutamate-induced neurotoxicity in primary cultured cortical neurons.
Short and long access to cocaine self-administration activates tyrosine phosphatase STEP and attenuates GluN expression but differentially regulates GluA expression in the prefrontal cortex.
Neuroprotective effects of Polygonum multiflorum extract against glutamate-induced oxidative toxicity in HT22 hippocampal cells.
Neuroprotective role of a brain-enriched tyrosine phosphatase, STEP, in focal cerebral ischemia.
Distinct roles for μ-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration.
Hippocampal CA3 and CA2 have distinct bilateral innervation patterns to CA1 in rodents.
Regulator of calmodulin signaling knockout mice display anxiety-like behavior and motivational deficits.
GluA2 AMPA glutamate receptor subunit exhibits codon 607 Q/R RNA editing in the lens.
Dephosphorylation of specific sites in the kinase-specificity sequence domain leads to ubiquitin-mediated degradation of the tyrosine phosphatase STEP.
Genetic reduction of striatal-enriched tyrosine phosphatase (STEP) reverses cognitive and cellular deficits in an Alzheimer's disease mouse model.
NR2B-NMDA receptor mediated modulation of the tyrosine phosphatase STEP regulates glutamate induced neuronal cell death.
Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61.
A role for calmodulin-stimulated adenylyl cyclases in cocaine sensitization.
Phospho-regulation of synaptic and extrasynaptic N-methyl-d-aspartate receptors in adult hippocampal slices.
The tyrosine phosphatase STEP mediates AMPA receptor endocytosis after metabotropic glutamate receptor stimulation.
Translation of striatal-enriched protein tyrosine phosphatase (STEP) after beta1-adrenergic receptor stimulation.
Synaptic plasticity (and the lack thereof) in hippocampal CA2 neurons.
Status epilepticus-induced somatostatinergic hilar interneuron degeneration is regulated by striatal enriched protein tyrosine phosphatase.
NMDA-mediated activation of the tyrosine phosphatase STEP regulates the duration of ERK signaling.
Tyrosine phosphatase STEP is a tonic brake on induction of long-term potentiation.
Tyrosine phosphatase STEP is a tonic brake on induction of long-term potentiation.
Striatal enriched phosphatase 61 dephosphorylates Fyn at phosphotyrosine 420.
The Dopamine/D1 receptor mediates the phosphorylation and inactivation of the protein tyrosine phosphatase STEP via a PKA-dependent pathway.
Calcium-dependent cleavage of striatal enriched tyrosine phosphatase (STEP).
Hypoxia-ischemia in perinatal rat brain induces the formation of a low molecular weight isoform of striatal enriched tyrosine phosphatase (STEP).
STEP61: a member of a family of brain-enriched PTPs is localized to the endoplasmic reticulum.
Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase.
Hu P, Liu J, Maita I, Kwok C, Gu E, Gergues MM, Kelada F, Phan M, Zhou JN, Swaab DF, Pang ZP, Lucassen PJ, Roepke TA, Samuels BA
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Mar 18;40(12):2519-2537
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Mar 18;40(12):2519-2537
Selective reduction of APP-BACE1 activity improves memory via NMDA-NR2B receptor-mediated mechanisms in aged PDAPP mice.
Evans CE, Thomas RS, Freeman TJ, Hvoslef-Eide M, Good MA, Kidd EJ
Neurobiology of aging 2019 Mar;75:136-149
Neurobiology of aging 2019 Mar;75:136-149
Age-related NMDA signaling alterations in SOD2 deficient mice.
Carvajal FJ, Mira RG, Rovegno M, Minniti AN, Cerpa W
Biochimica et biophysica acta. Molecular basis of disease 2018 Jun;1864(6 Pt A):2010-2020
Biochimica et biophysica acta. Molecular basis of disease 2018 Jun;1864(6 Pt A):2010-2020
Excitotoxic inactivation of constitutive oxidative stress detoxification pathway in neurons can be rescued by PKD1.
Pose-Utrilla J, García-Guerra L, Del Puerto A, Martín A, Jurado-Arjona J, De León-Reyes NS, Gamir-Morralla A, Sebastián-Serrano Á, García-Gallo M, Kremer L, Fielitz J, Ireson C, Pérez-Álvarez MJ, Ferrer I, Hernández F, Ávila J, Lasa M, Campanero MR, Iglesias T
Nature communications 2017 Dec 22;8(1):2275
Nature communications 2017 Dec 22;8(1):2275
Aging is associated with dimerization and inactivation of the brain-enriched tyrosine phosphatase STEP.
Rajagopal S, Deb I, Poddar R, Paul S
Neurobiology of aging 2016 May;41:25-38
Neurobiology of aging 2016 May;41:25-38
Zn2+-dependent Activation of the Trk Signaling Pathway Induces Phosphorylation of the Brain-enriched Tyrosine Phosphatase STEP: MOLECULAR BASIS FOR ZN2+-INDUCED ERK MAPK ACTIVATION.
Poddar R, Rajagopal S, Shuttleworth CW, Paul S
The Journal of biological chemistry 2016 Jan 8;291(2):813-25
The Journal of biological chemistry 2016 Jan 8;291(2):813-25
PSD-95 stabilizes NMDA receptors by inducing the degradation of STEP61.
Won S, Incontro S, Nicoll RA, Roche KW
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 9;113(32):E4736-44
Proceedings of the National Academy of Sciences of the United States of America 2016 Aug 9;113(32):E4736-44
A single fraction from Uncaria sinensis exerts neuroprotective effects against glutamate-induced neurotoxicity in primary cultured cortical neurons.
Kim HN, Jang JY, Choi BT
Anatomy & cell biology 2015 Jun;48(2):95-103
Anatomy & cell biology 2015 Jun;48(2):95-103
Short and long access to cocaine self-administration activates tyrosine phosphatase STEP and attenuates GluN expression but differentially regulates GluA expression in the prefrontal cortex.
Sun WL, Zelek-Molik A, McGinty JF
Psychopharmacology 2013 Oct;229(4):603-13
Psychopharmacology 2013 Oct;229(4):603-13
Neuroprotective effects of Polygonum multiflorum extract against glutamate-induced oxidative toxicity in HT22 hippocampal cells.
Kim HN, Kim YR, Jang JY, Choi YW, Baek JU, Hong JW, Choi YH, Shin HK, Choi BT
Journal of ethnopharmacology 2013 Oct 28;150(1):108-15
Journal of ethnopharmacology 2013 Oct 28;150(1):108-15
Neuroprotective role of a brain-enriched tyrosine phosphatase, STEP, in focal cerebral ischemia.
Deb I, Manhas N, Poddar R, Rajagopal S, Allan AM, Lombroso PJ, Rosenberg GA, Candelario-Jalil E, Paul S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Nov 6;33(45):17814-26
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Nov 6;33(45):17814-26
Distinct roles for μ-calpain and m-calpain in synaptic NMDAR-mediated neuroprotection and extrasynaptic NMDAR-mediated neurodegeneration.
Wang Y, Briz V, Chishti A, Bi X, Baudry M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Nov 27;33(48):18880-92
The Journal of neuroscience : the official journal of the Society for Neuroscience 2013 Nov 27;33(48):18880-92
Hippocampal CA3 and CA2 have distinct bilateral innervation patterns to CA1 in rodents.
Shinohara Y, Hosoya A, Yahagi K, Ferecskó AS, Yaguchi K, Sík A, Itakura M, Takahashi M, Hirase H
The European journal of neuroscience 2012 Mar;35(5):702-10
The European journal of neuroscience 2012 Mar;35(5):702-10
Regulator of calmodulin signaling knockout mice display anxiety-like behavior and motivational deficits.
Davis MM, Olausson P, Greengard P, Taylor JR, Nairn AC
The European journal of neuroscience 2012 Jan;35(2):300-8
The European journal of neuroscience 2012 Jan;35(2):300-8
GluA2 AMPA glutamate receptor subunit exhibits codon 607 Q/R RNA editing in the lens.
Farooq M, Kaswala RH, Kleiman NJ, Kasinathan C, Frederikse PH
Biochemical and biophysical research communications 2012 Feb 10;418(2):273-7
Biochemical and biophysical research communications 2012 Feb 10;418(2):273-7
Dephosphorylation of specific sites in the kinase-specificity sequence domain leads to ubiquitin-mediated degradation of the tyrosine phosphatase STEP.
Mukherjee S, Poddar R, Deb I, Paul S
The Biochemical journal 2011 Nov 15;440(1):115-25
The Biochemical journal 2011 Nov 15;440(1):115-25
Genetic reduction of striatal-enriched tyrosine phosphatase (STEP) reverses cognitive and cellular deficits in an Alzheimer's disease mouse model.
Zhang Y, Kurup P, Xu J, Carty N, Fernandez SM, Nygaard HB, Pittenger C, Greengard P, Strittmatter SM, Nairn AC, Lombroso PJ
Proceedings of the National Academy of Sciences of the United States of America 2010 Nov 2;107(44):19014-9
Proceedings of the National Academy of Sciences of the United States of America 2010 Nov 2;107(44):19014-9
NR2B-NMDA receptor mediated modulation of the tyrosine phosphatase STEP regulates glutamate induced neuronal cell death.
Poddar R, Deb I, Mukherjee S, Paul S
Journal of neurochemistry 2010 Dec;115(6):1350-62
Journal of neurochemistry 2010 Dec;115(6):1350-62
Abeta-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61.
Kurup P, Zhang Y, Xu J, Venkitaramani DV, Haroutunian V, Greengard P, Nairn AC, Lombroso PJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Apr 28;30(17):5948-57
The Journal of neuroscience : the official journal of the Society for Neuroscience 2010 Apr 28;30(17):5948-57
A role for calmodulin-stimulated adenylyl cyclases in cocaine sensitization.
DiRocco DP, Scheiner ZS, Sindreu CB, Chan GC, Storm DR
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 25;29(8):2393-403
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 25;29(8):2393-403
Phospho-regulation of synaptic and extrasynaptic N-methyl-d-aspartate receptors in adult hippocampal slices.
Goebel-Goody SM, Davies KD, Alvestad Linger RM, Freund RK, Browning MD
Neuroscience 2009 Feb 18;158(4):1446-59
Neuroscience 2009 Feb 18;158(4):1446-59
The tyrosine phosphatase STEP mediates AMPA receptor endocytosis after metabotropic glutamate receptor stimulation.
Zhang Y, Venkitaramani DV, Gladding CM, Zhang Y, Kurup P, Molnar E, Collingridge GL, Lombroso PJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 15;28(42):10561-6
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Oct 15;28(42):10561-6
Translation of striatal-enriched protein tyrosine phosphatase (STEP) after beta1-adrenergic receptor stimulation.
Hu Y, Zhang Y, Venkitaramani DV, Lombroso PJ
Journal of neurochemistry 2007 Oct;103(2):531-41
Journal of neurochemistry 2007 Oct;103(2):531-41
Synaptic plasticity (and the lack thereof) in hippocampal CA2 neurons.
Zhao M, Choi YS, Obrietan K, Dudek SM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 31;27(44):12025-32
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Oct 31;27(44):12025-32
Status epilepticus-induced somatostatinergic hilar interneuron degeneration is regulated by striatal enriched protein tyrosine phosphatase.
Choi YS, Lin SL, Lee B, Kurup P, Cho HY, Naegele JR, Lombroso PJ, Obrietan K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Mar 14;27(11):2999-3009
The Journal of neuroscience : the official journal of the Society for Neuroscience 2007 Mar 14;27(11):2999-3009
NMDA-mediated activation of the tyrosine phosphatase STEP regulates the duration of ERK signaling.
Paul S, Nairn AC, Wang P, Lombroso PJ
Nature neuroscience 2003 Jan;6(1):34-42
Nature neuroscience 2003 Jan;6(1):34-42
Tyrosine phosphatase STEP is a tonic brake on induction of long-term potentiation.
Pelkey KA, Askalan R, Paul S, Kalia LV, Nguyen TH, Pitcher GM, Salter MW, Lombroso PJ
Neuron 2002 Mar 28;34(1):127-38
Neuron 2002 Mar 28;34(1):127-38
Tyrosine phosphatase STEP is a tonic brake on induction of long-term potentiation.
Pelkey KA, Askalan R, Paul S, Kalia LV, Nguyen TH, Pitcher GM, Salter MW, Lombroso PJ
Neuron 2002 Mar 28;34(1):127-38
Neuron 2002 Mar 28;34(1):127-38
Striatal enriched phosphatase 61 dephosphorylates Fyn at phosphotyrosine 420.
Nguyen TH, Liu J, Lombroso PJ
The Journal of biological chemistry 2002 Jul 5;277(27):24274-9
The Journal of biological chemistry 2002 Jul 5;277(27):24274-9
The Dopamine/D1 receptor mediates the phosphorylation and inactivation of the protein tyrosine phosphatase STEP via a PKA-dependent pathway.
Paul S, Snyder GL, Yokakura H, Picciotto MR, Nairn AC, Lombroso PJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2000 Aug 1;20(15):5630-8
The Journal of neuroscience : the official journal of the Society for Neuroscience 2000 Aug 1;20(15):5630-8
Calcium-dependent cleavage of striatal enriched tyrosine phosphatase (STEP).
Nguyen TH, Paul S, Xu Y, Gurd JW, Lombroso PJ
Journal of neurochemistry 1999 Nov;73(5):1995-2001
Journal of neurochemistry 1999 Nov;73(5):1995-2001
Hypoxia-ischemia in perinatal rat brain induces the formation of a low molecular weight isoform of striatal enriched tyrosine phosphatase (STEP).
Gurd JW, Bissoon N, Nguyen TH, Lombroso PJ, Rider CC, Beesley PW, Vannucci SJ
Journal of neurochemistry 1999 Nov;73(5):1990-4
Journal of neurochemistry 1999 Nov;73(5):1990-4
STEP61: a member of a family of brain-enriched PTPs is localized to the endoplasmic reticulum.
Bult A, Zhao F, Dirkx R Jr, Sharma E, Lukacsi E, Solimena M, Naegele JR, Lombroso PJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 1996 Dec 15;16(24):7821-31
The Journal of neuroscience : the official journal of the Society for Neuroscience 1996 Dec 15;16(24):7821-31
Cellular and molecular characterization of a brain-enriched protein tyrosine phosphatase.
Boulanger LM, Lombroso PJ, Raghunathan A, During MJ, Wahle P, Naegele JR
The Journal of neuroscience : the official journal of the Society for Neuroscience 1995 Feb;15(2):1532-44
The Journal of neuroscience : the official journal of the Society for Neuroscience 1995 Feb;15(2):1532-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STEP Antibody (23E5) [NB300-202] - Analysis of STEP protein in striatal rat protein homogenates
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STEP Antibody (23E5) [NB300-202] - STEP was detected in immersion fixed paraffin-embedded sections of human brain basal ganglia using Mouse Anti-Human STEP (23E5) Monoclonal Antibody (Catalog # NB300-202) at 1:100 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in neurons.
