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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF7109 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal NELL1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse NELL1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human NELL1 is observed and approximately 10% cross-reactivity with recombinant mouse NELL2 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse NELL1 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse NELL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7109) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NELL1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- NELL1 in Mouse Embryo. NELL1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse NELL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7109) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in the spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
