H00008027-M01

antibody from Abnova Corporation

Targeting: STAM STAM1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00008027-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00008027-M01, RRID:AB_425755

Product name

STAM monoclonal antibody (M01), clone 2B11-1G1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant STAM.

Antigen sequence

MPLFATNPFDQDVEKATSEMNTAEDWGLILDICDK
VGQSRTGPKDCLRSIMRRVNHKDPHVAMQALTLLG
ACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVC
EKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGV
TFPAIGSQAAEQAKASPALVAKDPGTVANKKEEED
LAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQH
EGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDP
NWWKGETHQGIGLFPSNFVTAYLTAEPEMIKTEKK
TVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQ
STDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDI
DRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYA
KLQNQPGSGPTIRKPSPS


Isotype

IgG

Antibody clone number

2B11-1G1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of STAM expression in transfected 293T cell line by STAM monoclonal antibody (M01), clone 2B11-1G1.Lane 1: STAM transfected lysate(45 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged STAM is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of STAM transfected lysate using anti-STAM monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with STAM monoclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to STAM on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol