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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF3245a - Provider product page
- Provider
- Abgent
- Product name
- FANCF (aa217-228) Antibody (internal region)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide-affinity purified goat antibody
- Reactivity
- Mouse, Bovine, Canine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1mg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -953°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
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- Abgent (provider)
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- Experimental details
- HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with AF3245a (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with AF3245a (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
