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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10011 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10011, RRID:AB_1674783
- Product name
- FANCF polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FANCF.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The Fanconi anemia gene product FANCF is a flexible adaptor protein.
Promoter hypermethylation of FANCF: disruption of Fanconi Anemia-BRCA pathway in cervical cancer.
Inactivation of the Fanconi anemia/BRCA pathway in lung and oral cancers: implications for treatment and survival.
Léveillé F, Blom E, Medhurst AL, Bier P, Laghmani el H, Johnson M, Rooimans MA, Sobeck A, Waisfisz Q, Arwert F, Patel KJ, Hoatlin ME, Joenje H, de Winter JP
The Journal of biological chemistry 2004 Sep 17;279(38):39421-30
The Journal of biological chemistry 2004 Sep 17;279(38):39421-30
Promoter hypermethylation of FANCF: disruption of Fanconi Anemia-BRCA pathway in cervical cancer.
Narayan G, Arias-Pulido H, Nandula SV, Basso K, Sugirtharaj DD, Vargas H, Mansukhani M, Villella J, Meyer L, Schneider A, Gissmann L, Dürst M, Pothuri B, Murty VV
Cancer research 2004 May 1;64(9):2994-7
Cancer research 2004 May 1;64(9):2994-7
Inactivation of the Fanconi anemia/BRCA pathway in lung and oral cancers: implications for treatment and survival.
Marsit CJ, Liu M, Nelson HH, Posner M, Suzuki M, Kelsey KT
Oncogene 2004 Jan 29;23(4):1000-4
Oncogene 2004 Jan 29;23(4):1000-4
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using FANCF polyclonal antibody (Cat # PAB10011) shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4).This band (indicated by arrowhead) is approximately 42.3 kDa in size.The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2).Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation.No apparent difference was noted upon irradiation.The strong band at ~60kDa appears to be non-specific.Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
