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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 600-401-674 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-674, RRID:AB_2293833
- Product name
- Anti-FANCF (RABBIT) Antibody - 600-401-674
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
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- Experimental details
- Western blot using Rockland's affinity purified anti-FANCF antibody shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4). This band (indicated by arrowhead) is approximately 42.3 kDa in size. The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2). Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation. No apparent difference was noted upon irradiation. The strong band at ~60kDa appears to be non-specific. Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
