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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- OAEB01032 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#OAEB01032, RRID:AB_10883015
- Product name
- FANCF Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Goat
- Vial size
- 0.1mg
- Storage
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
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- Aviva Systems Biology (provider)
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- Experimental details
- HEK293 lysate (10µg protein in RIPA buffer) overexpressing Human FANCF with C-terminal MYC tag probed with OAEB01032 (0.5µg/ml ) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with OAEB01032 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
