PA5-117901

antibody from Invitrogen Antibodies

Targeting: CYB5R1 humb5R2, NQO3A2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117901

Provider

Invitrogen Antibodies

Product name

CYB5R1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CYB5R1 Polyclonal Antibody (Product # PA5-117901) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate, Lane C: A549 Whole Cell Lysate, Lane D: COS7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa. (We are unsure of the identity of these extra bands).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CYB5R1 Polyclonal Antibody (Product # PA5-117901) at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate, Lane C: A549 Whole Cell Lysate, Lane D: COS7 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CYB5R1 in human kidney with CYB5R1 Polyclonal Antibody (Product # PA5-117901, 1:100 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human CYB5R1 in human skeletal muscle with CYB5R1 Polyclonal Antibody (Product # PA5-117901, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CYB5R1 Immunoprecipitation using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 4 µL with CYB5R1 Polyclonal Antibody (Product # PA5-117901) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CYB5R1 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa. Observed band size: 34 kDa.