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- Antibody Data
- Antigen structure
- References [28]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- 14643-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14643-1-AP, RRID:AB_2178440
- Product name
- REEP5 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated REEP5 antibody (Cat. #14643-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references ATF4 links ER stress with reticulophagy in glioblastoma cells.
A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells.
Mechanism of membrane-curvature generation by ER-tubule shaping proteins.
Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.
A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.
Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190.
DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.
A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.
REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects.
Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.
ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.
TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.
REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis.
Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.
Combination anti-Aβ treatment maximizes cognitive recovery and rebalances mTOR signaling in APP mice.
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae.
Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.
Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.
Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.
Molecular Mechanisms of Disease Pathogenesis Differ in Krabbe Disease Variants.
ER network formation and membrane fusion by atlastin1/SPG3A disease variants.
A high-content screening microscopy approach to dissect the role of Rab proteins in Golgi-to-ER retrograde trafficking.
Spastin binds to lipid droplets and affects lipid metabolism.
Pharmacologic rescue of axon growth defects in a human iPSC model of hereditary spastic paraplegia SPG3A.
Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Zielke S, Kardo S, Zein L, Mari M, Covarrubias-Pinto A, Kinzler MN, Meyer N, Stolz A, Fulda S, Reggiori F, Kögel D, van Wijk S
Autophagy 2021 Sep;17(9):2432-2448
Autophagy 2021 Sep;17(9):2432-2448
A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells.
Garcia-Pardo ME, Simpson JC, O'Sullivan NC
BMC bioinformatics 2021 Sep 8;22(1):427
BMC bioinformatics 2021 Sep 8;22(1):427
Mechanism of membrane-curvature generation by ER-tubule shaping proteins.
Wang N, Clark LD, Gao Y, Kozlov MM, Shemesh T, Rapoport TA
Nature communications 2021 Jan 25;12(1):568
Nature communications 2021 Jan 25;12(1):568
Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.
Wang X, Wang H, Xu B, Huang D, Nie C, Pu L, Zajac GJM, Yan H, Zhao J, Shi F, Emmer BT, Lu J, Wang R, Dong X, Dai J, Zhou W, Wang C, Gao G, Wang Y, Willer C, Lu X, Zhu Y, Chen XW
Cell metabolism 2021 Feb 2;33(2):350-366.e7
Cell metabolism 2021 Feb 2;33(2):350-366.e7
A proteome-wide map of 20(S)-hydroxycholesterol interactors in cell membranes.
Cheng YS, Zhang T, Ma X, Pratuangtham S, Zhang GC, Ondrus AA, Mafi A, Lomenick B, Jones JJ, Ondrus AE
Nature chemical biology 2021 Dec;17(12):1271-1280
Nature chemical biology 2021 Dec;17(12):1271-1280
Physical and Functional Analysis of the Putative Rpn13 Inhibitor RA190.
Dickson P, Abegg D, Vinogradova E, Takaya J, An H, Simanski S, Cravatt BF, Adibekian A, Kodadek T
Cell chemical biology 2020 Nov 19;27(11):1371-1382.e6
Cell chemical biology 2020 Nov 19;27(11):1371-1382.e6
DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.
Tian X, Zheng P, Zhou C, Wang X, Ma H, Ma W, Zhou X, Teng J, Chen J
Autophagy 2020 May;16(5):797-810
Autophagy 2020 May;16(5):797-810
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610
Scientific reports 2020 May 6;10(1):7610
Functional culture and in vitro genetic and small-molecule manipulation of adult mouse cardiomyocytes.
Callaghan NI, Lee SH, Hadipour-Lakmehsari S, Lee XA, Ahsan Siraj M, Driouchi A, Yip CM, Husain M, Simmons CA, Gramolini AO
Communications biology 2020 May 11;3(1):229
Communications biology 2020 May 11;3(1):229
A Genome-wide ER-phagy Screen Highlights Key Roles of Mitochondrial Metabolism and ER-Resident UFMylation.
Liang JR, Lingeman E, Luong T, Ahmed S, Muhar M, Nguyen T, Olzmann JA, Corn JE
Cell 2020 Mar 19;180(6):1160-1177.e20
Cell 2020 Mar 19;180(6):1160-1177.e20
REEP5 depletion causes sarco-endoplasmic reticulum vacuolization and cardiac functional defects.
Lee SH, Hadipour-Lakmehsari S, Murthy HR, Gibb N, Miyake T, Teng ACT, Cosme J, Yu JC, Moon M, Lim S, Wong V, Liu P, Billia F, Fernandez-Gonzalez R, Stagljar I, Sharma P, Kislinger T, Scott IC, Gramolini AO
Nature communications 2020 Feb 19;11(1):965
Nature communications 2020 Feb 19;11(1):965
Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.
Sharoar MG, Hu X, Ma XM, Zhu X, Yan R
Molecular psychiatry 2019 Sep;24(9):1369-1382
Molecular psychiatry 2019 Sep;24(9):1369-1382
ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.
Chen Q, Xiao Y, Chai P, Zheng P, Teng J, Chen J
Current biology : CB 2019 Mar 4;29(5):846-855.e6
Current biology : CB 2019 Mar 4;29(5):846-855.e6
TEX264 Is an Endoplasmic Reticulum-Resident ATG8-Interacting Protein Critical for ER Remodeling during Nutrient Stress.
An H, Ordureau A, Paulo JA, Shoemaker CJ, Denic V, Harper JW
Molecular cell 2019 Jun 6;74(5):891-908.e10
Molecular cell 2019 Jun 6;74(5):891-908.e10
REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis.
Kumar D, Golchoubian B, Belevich I, Jokitalo E, Schlaitz AL
Molecular biology of the cell 2019 Jun 1;30(12):1377-1389
Molecular biology of the cell 2019 Jun 1;30(12):1377-1389
Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.
Li L, Wu J, Cao X, Zhou J, Yin S, Yang S, Feng Q, Du P, Liu Y, Shang Y, Liu Y, Liu X
Research in veterinary science 2019 Aug;125:195-204
Research in veterinary science 2019 Aug;125:195-204
Combination anti-Aβ treatment maximizes cognitive recovery and rebalances mTOR signaling in APP mice.
Chiang ACA, Fowler SW, Savjani RR, Hilsenbeck SG, Wallace CE, Cirrito JR, Das P, Jankowsky JL
The Journal of experimental medicine 2018 May 7;215(5):1349-1364
The Journal of experimental medicine 2018 May 7;215(5):1349-1364
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Zheng P, Chen Q, Tian X, Qian N, Chai P, Liu B, Hu J, Blackstone C, Zhu D, Teng J, Chen J
Cell research 2018 Aug;28(8):833-854
Cell research 2018 Aug;28(8):833-854
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae.
Wang X, Li S, Wang H, Shui W, Hu J
eLife 2017 Mar 13;6
eLife 2017 Mar 13;6
Full length RTN3 regulates turnover of tubular endoplasmic reticulum via selective autophagy.
Grumati P, Morozzi G, Hölper S, Mari M, Harwardt MI, Yan R, Müller S, Reggiori F, Heilemann M, Dikic I
eLife 2017 Jun 15;6
eLife 2017 Jun 15;6
Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.
Sharoar MG, Shi Q, Ge Y, He W, Hu X, Perry G, Zhu X, Yan R
Molecular psychiatry 2016 Sep;21(9):1263-71
Molecular psychiatry 2016 Sep;21(9):1263-71
Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.
Renvoisé B, Malone B, Falgairolle M, Munasinghe J, Stadler J, Sibilla C, Park SH, Blackstone C
Human molecular genetics 2016 Dec 1;25(23):5111-5125
Human molecular genetics 2016 Dec 1;25(23):5111-5125
Molecular Mechanisms of Disease Pathogenesis Differ in Krabbe Disease Variants.
Spratley SJ, Hill CH, Viuff AH, Edgar JR, Skjødt K, Deane JE
Traffic (Copenhagen, Denmark) 2016 Aug;17(8):908-22
Traffic (Copenhagen, Denmark) 2016 Aug;17(8):908-22
ER network formation and membrane fusion by atlastin1/SPG3A disease variants.
Ulengin I, Park JJ, Lee TH
Molecular biology of the cell 2015 May 1;26(9):1616-28
Molecular biology of the cell 2015 May 1;26(9):1616-28
A high-content screening microscopy approach to dissect the role of Rab proteins in Golgi-to-ER retrograde trafficking.
Galea G, Bexiga MG, Panarella A, O'Neill ED, Simpson JC
Journal of cell science 2015 Jul 1;128(13):2339-49
Journal of cell science 2015 Jul 1;128(13):2339-49
Spastin binds to lipid droplets and affects lipid metabolism.
Papadopoulos C, Orso G, Mancuso G, Herholz M, Gumeni S, Tadepalle N, Jüngst C, Tzschichholz A, Schauss A, Höning S, Trifunovic A, Daga A, Rugarli EI
PLoS genetics 2015 Apr;11(4):e1005149
PLoS genetics 2015 Apr;11(4):e1005149
Pharmacologic rescue of axon growth defects in a human iPSC model of hereditary spastic paraplegia SPG3A.
Zhu PP, Denton KR, Pierson TM, Li XJ, Blackstone C
Human molecular genetics 2014 Nov 1;23(21):5638-48
Human molecular genetics 2014 Nov 1;23(21):5638-48
Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
Chang J, Lee S, Blackstone C
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 10;110(37):14954-9
Proceedings of the National Academy of Sciences of the United States of America 2013 Sep 10;110(37):14954-9
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- human liver tissue were subjected to SDS PAGE followed by western blot with 14643-1-AP(REEP5 antibody) at dilution of 1:500
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-REEP5 (IP:14643-1-AP, 3ug; Detection:14643-1-AP 1:1000) with HeLa cells lysate 2400ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human colon cancer using 14643-1-AP(REEP5 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The REEP5 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human REEP5. This antibody recognizes human,mouse,rat antigen. The REEP5 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
