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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 19569-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIAA0494 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MGLNKVWIN ITEMNKQISL LTSAVNHLKA NVKSAADLIS LPTTVEGLQK SVASIGNTLN SVHLAVEALQ KTVDEHKKTM ELLQSDMNQH FLKETPGSNQ IIPSPSATSE LDNKTHSENL KQDILYLHNS LEEVNSALVG YQRQNDLKLE GMNETVSNLT QRVNLIESDV VAMSKVEKKA NLSFSMMGDR SATLKRQSLD QVTNRTDTVK IQSIKKEDSS NSQVSKLREK LQLISALTNK PESNRPPETA DEEQVESFTS KPSALPKFSQ FLGDPVEKAA QLRPISLPGV SSTEDLQDLF RKTGQDVDGK LTYQEIWTSL GSAMPEPESL RAFDSDGDGR YSFLELRVAL GI (145-495 aa encoded by BC002525)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.26 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with (Product # 19569-1-AP) (KIAA0494 antibody) at dilution of 1:4,000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 19569-1-AP ( KIAA0494 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 19569-1-AP (KIAA0494 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 19569-1-AP (KIAA0494 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 19569-1-AP (KIAA0494 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 19569-1-AP ( KIAA0494 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human stomach tissue slide using 19569-1-AP ( KIAA0494 Antibody) at dilution of 1:50 (under 40x lens).
