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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP10208c - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP10208c, RRID:AB_10819136
- Product name
- SPARCL1 Antibody (Center)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SPARCL1 Antibody (Center) (Cat. #AP10208c) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SPARCL1 antibody detected the SPARCL1 protein (arrow).
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- SPARCL1 antibody (Center) (Cat. #AP10208c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPARCL1 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:50~100
