AF2728

antibody from Novus Biologicals

Targeting: SPARCL1 MAST9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2728

Provider

Novus Biologicals

Product name

Goat Polyclonal SPARC-like 1/SPARCL1 Antibody

Antibody type

Polyclonal

References

Submitted references

SPARCL1, a Novel Prognostic Predictive Factor for GI Malignancies: a Meta-Analysis.

Hu H, Cai W, Zheng S, Ge W
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;44(4):1485-1496

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Molecular resistance fingerprint of pemetrexed and platinum in a long-term survivor of mesothelioma.

Røe OD, Szulkin A, Anderssen E, Flatberg A, Sandeck H, Amundsen T, Erlandsen SE, Dobra K, Sundstrøm SH
PloS one 2012;7(8):e40521

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Down-regulated SPARCL1 is associated with clinical significance in human gastric cancer.

Li P, Qian J, Yu G, Chen Y, Liu K, Li J, Wang J
Journal of surgical oncology 2012 Jan;105(1):31-7

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human SPARC-like 1/SPARCL1 by Western Blot. Western blot shows lysates of human lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SPARC-like 1/ SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC-like 1/SPARCL1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

SPARC-like 1/SPARCL1 in Human Brain. SPARC-like 1/SPARCL1 was detected in immersion fixed paraffin-embedded sections of human brain using Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of SPARC-like 1/SPARCL1 in HL-60 Human Cell Line by Flow Cytometry. HL-60 human acute promyelocytic leukemia cell line was stained with Goat Anti-Human SPARC-like 1/SPARCL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2728, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.