13517-1-AP

antibody from Invitrogen Antibodies

Targeting: SPARCL1 MAST9

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13517-1-AP

Provider

Invitrogen Antibodies

Product name

SPARCL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VSEALLMEP TDDGNTTPRN HGVDDDGDDD GDDGGTDGPR HSASDDYFIP SQAFLEAERA QSIAYHLKIE EQREKVHENE NIGTTEPGEH QEAKKAENSS NEEETSSEGN MRVHAVDSCM SFQCKRGHIC KADQQGKPHC VCQDPVTCPP TKPLDQVCGT DNQTYASSCH LFATKCRLEG TKKGHQLQLD YFGACKSIPT CTDFEVIQFP LRMRDWLKNI LMQLYEANSE HAGYLNEKQR NKVKKIYLDE KRLLAGDHPI DLLLRDFKKN YHMYVYPVHW QFSELDQHPM DRVLTHSELA PLRASLVPME HCITRFFEEC DPNKDKHITL KEWGHCFGIK EEDIDENLLF (316-664 aa encoded by BC033721)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.31 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human heart tissue were subjected to SDS PAGE followed by western blot with 13517-1-AP (SPARCL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 13517-1-AP (SPARCL1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 13517-1-AP (SPARCL1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SPARCL1 (IP:13517-1-AP, 4ug; Detection:13517-1-AP 1:300) with Raji cells lysate 1440ug.