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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Other assay [2]
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- Product number
- PA5-44359 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATP6V0D2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide sequence: GLRLLAQAED FDQMKNVADH YGVYKPLFEA VGGSGGKTLE DVFYEREVQM
- Concentration
- 0.5 mg/mL
Submitted references Osteoclast fusion and bone loss are restricted by interferon inducible guanylate binding proteins.
A20 inhibits osteoclastogenesis via TRAF6-dependent autophagy in human periodontal ligament cells under hypoxia.
A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.
Place DE, Malireddi RKS, Kim J, Vogel P, Yamamoto M, Kanneganti TD
Nature communications 2021 Jan 21;12(1):496
Nature communications 2021 Jan 21;12(1):496
A20 inhibits osteoclastogenesis via TRAF6-dependent autophagy in human periodontal ligament cells under hypoxia.
Yan K, Wu C, Ye Y, Li L, Wang X, He W, Ren S, Xu Y
Cell proliferation 2020 Mar;53(3):e12778
Cell proliferation 2020 Mar;53(3):e12778
A revised airway epithelial hierarchy includes CFTR-expressing ionocytes.
Montoro DT, Haber AL, Biton M, Vinarsky V, Lin B, Birket SE, Yuan F, Chen S, Leung HM, Villoria J, Rogel N, Burgin G, Tsankov AM, Waghray A, Slyper M, Waldman J, Nguyen L, Dionne D, Rozenblatt-Rosen O, Tata PR, Mou H, Shivaraju M, Bihler H, Mense M, Tearney GJ, Rowe SM, Engelhardt JF, Regev A, Rajagopal J
Nature 2018 Aug;560(7718):319-324
Nature 2018 Aug;560(7718):319-324
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of transfected 293T cells using an anti-ATP6V0D2 polyclonal antibody (Product # PA5-44359).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 A20 inhibits osteoclastogenesis through downregulating autophagy under hypoxia. A, After becoming pre-osteoclasts, BMMCs were cultured in CM from cells transfected with lentivirus A20 for overexpression. Osteoclast differentiation was measured by TRAP staining. B, The cytoskeleton of multinuclear cells was detected by IF (bar = 100 mum). C, The areas of hydroxyapatite resorption were measured by toluidine blue staining (bar = 100 mum). D, The expression of osteoclast-related factors (CTSK, MMP9, V-ATPase D2 and TRAP) in BMMCs was detected by Western blot. Data are presented as mean +- SD, ** P < .01, *** P < .001
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 A20 inhibits osteoclastogenesis through downregulating autophagy under hypoxia. A, After becoming pre-osteoclasts, BMMCs were cultured in CM from cells transfected with lentivirus A20 for silencing. Osteoclast differentiation was measured by TRAP staining. B, The cytoskeleton of multinuclear cells was detected by IF (bar = 100 mum). C, The areas of hydroxyapatite resorption were measured by toluidine blue staining (bar = 100 mum). D, The expression of osteoclast-related factors (CTSK, MMP9, V-ATPase D2 and TRAP) in BMMCs was detected by Western blot. Data are presented as mean +- SD, * P < .05, ** P < .01, *** P < .001
