Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 17495-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPAG4L Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MPRSSRSPG DPGALLEDVA HNPRPRRIAQ RGRNTSRMAE DTSPNMNDNI LLPVRNNDQA LGLTQCMLGC VSWFTCFACS LRTQAQQVLF NTCRCKLLCQ KLMEKTGILL LCAFGFWMFS IHLPSKMKVW QDDSINGPLQ SLRLYQEKVR HHSGEIQDLR GSMNQLIAKL QEMEAMSDEQ KMAQKIMKMI HGDYIEKPDF ALKSIGASID FEHTSVTYNH EKAHSYWNWI QLWNYAQPPD VILEPNVTPG NCWAFEGDRG QVTIQLAQKV YLSNLTLQHI PKTISLSGSL DTAPKDFVIY GMEGSPKEEV FLGAFQFQPE NIIQMFPLQN QPARAFSAVK VKISSNWGNP GFTCLYRVRV HGSVAPPREQ PHQNPYPKRD (1-379 aa encoded by BC026118)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.24 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 17495-1-AP (SPAG4L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 17495-1-AP (SPAG4L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed PC-3 cells using 17495-1-AP (SPAG4L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).