H00006443-M02

antibody from Abnova Corporation

Targeting: SGCB A3b, LGMD2E, SGC

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006443-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006443-M02, RRID:AB_566180

Product name

SGCB monoclonal antibody (M02), clone 1C10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SGCB.

Antigen sequence

WAVIRIGPNGCDSMEFHESGLLRFKQVSDMGVIHP
LYKSTVGGRRNENLVITGNNQPIVFQQGTTKLSVE
NNKTSITSDIGMQFFDPRTQNILFSTDYETHEFHL
PSGVK


Isotype

IgG

Antibody clone number

1C10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

SGCB monoclonal antibody (M02), clone 1C10 Western Blot analysis of SGCB expression in MCF-7 ( Cat # L046V1 ).

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SGCB is 0.1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to SGCB on MCF-7 cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to SGCB on formalin-fixed paraffin-embedded human heart. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol