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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14921-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- THUMPD1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAPAQQTT QPGGGKRKGK AQYVLAKRAR RCDAGGPRQL EPGLQGILIT CNMNERKCVE EAYSLLNEYG DDMYGPEKFT DKDQQPSGSE GEDDDAEAAL KKEVGDIKAS TEMRLRRFQS VESGANNVVF IRTLGIEPEK LVHHILQDMY KTKKKKTRVI LRMLPISGTC KAFLEDMKKY AETFLEPWFK APNKGTFQIV YKSRNNSHVN REEVIRELAG IVCTLNSENK VDLTNPQYTV VVEIIKAVCC LSVVKDYMLF RKYNLQEVVK SPKDPSQLNS KQGNGKEAKL ESADKSDQNN TAEGKNNQQV PENTEELGQT KPTSNPQVVN EGGAKPELAS QATEGSKSNE NDFS (1-353 aa encoded by BC000448)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed MCF-7 cells using 14921-1-AP (THUMPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
