AF725
antibody from Novus Biologicals
Targeting: CCL27
ALP, CTACK, CTAK, ESkine, ILC, PESKY, SCYA27, skinkine
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF725 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal CCL27/CTACK Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL27/CTACK in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Comprehensive assessment of chemokine expression profiles by flow cytometry.
Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Trafficking pathways and characterization of CD4 and CD8 cells recruited to the skin of humans experimentally infected with Haemophilus ducreyi.
Humphreys TL, Baldridge LA, Billings SD, Campbell JJ, Spinola SM
Infection and immunity 2005 Jul;73(7):3896-902
Infection and immunity 2005 Jul;73(7):3896-902
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CCL27/CTACK in Mouse Skin. CCL27/CTACK was detected in perfusion fixed frozen sections of mouse skin using Goat Anti-Mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF725) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL27/CTACK and Neutral-ization by Mouse CCL27/ CTACK Antibody. Recombinant Mouse CCL27/CTACK (Catalog # 725-CK) chemo-attracts the BaF3 mouse pro-B cell line transfected with mouse CCR10 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Alamar blue staining. Chemotaxis elicited by Recombinant Mouse CCL27/CTACK (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL27/CTACK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF725). The ND50 is typically 10-20 µg/mL.