500-P294-100UG
antibody from Invitrogen Antibodies
Targeting: CCL27
ALP, CTACK, CTAK, ESkine, ILC, PESKY, SCYA27, skinkine
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- 500-P294-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL27 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: FLLPPSTACC TQLYRKPLSD KLLRKVIQVE LQEADGDCHL QAFVLHLAQR SICIHPQNPS LSQWFEHQER KLHGTLPKLN FGMLRKMG.
- Concentration
- 0.1-1.0 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human CTACK by Western Blot analysis CCL27 Polyclonal Antibody (Product # 500-P294-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CTACK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human CTACK (CCL27) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL27 Polyclonal Antibody (Product # 500-P294-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL27 Polyclonal Antibody, Biotin (Product # 500-P294BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CTACK (CCL27).