11792-1-AP

antibody from Invitrogen Antibodies

Targeting: RAB8B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11792-1-AP

Provider

Invitrogen Antibodies

Product name

RAB8B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MAKTYDYLF KLLLIGDSGV GKTCLLFRFS EDAFNTTFIS TIGIDFKIRT IELDGKKIKL QIWDTAGQER FRTITTAYYR GAMGIMLVYD ITNEKSFDNI KNWIRNIEEH ASSDVERMIL GNKCDMNDKR QVSKERGEKL AIDYGIKFLE TSAKSSANVE EAFFTLARDI MTKLNRKMND SNSAGAGGPV KITENRSKKT SFFRCSLL (1-207 aa encoded by BC020654)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 11792-1-AP (RAB8B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11792-1-AP (RAB8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11792-1-AP ( RAB8B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 11792-1-AP (RAB8B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11792-1-AP ( RAB8B antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Sodium Citrate buffer (pH6).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 11792-1-AP ( RAB8B antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Sodium Citrate buffer (pH6).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RAB8B (IP:11792-1-AP, 4ug; Detection:11792-1-AP 1:1000) with mouse brain tissue lysate 4000ug.