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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00001479-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001479-M01, RRID:AB_2261162
- Product name
- Mouse Monoclonal CSTF3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CSTF3 - cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Polyadenylation site-specific differences in the activity of the neuronal βCstF-64 protein in PC-12 cells.
Shankarling GS, MacDonald CC
Gene 2013 Oct 25;529(2):220-7
Gene 2013 Oct 25;529(2):220-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi ( Cat # H00001479-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody (M01), clone 1D4 (Cat # H00001479-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody (M01), clone 1D4. Lane 1: CSTF3 transfected lysate(11.44 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: CSTF3 Antibody (1D4) [H00001479-M01] - Detection limit for recombinant GST tagged CSTF3 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CSTF3 Antibody (1D4) [H00001479-M01] - Analysis of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
