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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- 701869 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KMT2D Recombinant Rabbit Monoclonal Antibody (9H13L17)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Monkey, Cat, Mouse and Sheep.
- Antibody clone number
- 9H13L17
- Concentration
- 0.5 mg/mL
Submitted references KDM1A maintains genome-wide homeostasis of transcriptional enhancers.
Agarwal S, Bonefas KM, Garay PM, Brookes E, Murata-Nakamura Y, Porter RS, Macfarlan TS, Ren B, Iwase S
Genome research 2021 Jan 7;31(2):186-97
Genome research 2021 Jan 7;31(2):186-97
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- For immunofluorescence analysis, U2OS cells were fixed and permeabilized for detection of endogenous KMT2D using Anti-KMT2D Recombinant Rabbit Monoclonal Antibody (Product # 701869, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of KMT2D protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of KMT2D. Panel e) shows untreated cells with no signal. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of endogenous KMT2D was performed on HEK-293 cells labeled with ABfinity™ Anti-KMT2D Recombinant Rabbit Monoclonal Antibody (Product# 701869, 5 ug/ 1M cells) or with rabbit isotype control at 0.5 ug/ml and detected with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, (Alexa Fluor® 488 conjugate, Product# A27034, 0.4 ug/ml, 1:2500) as represented by the red and pink histograms respectively. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control. A representative of 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer (4468770).
