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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-56772 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KIAA1429 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LSGRLDSDEQ KIQNDIIDIL LTFTQGVNEK LTISEETLAN NTWSLMLKEV LSSILKVPEG FFSGLILLSE LLPLPLPMQT TQVIEPHDIS VALNTRKL
- Concentration
- 0.10 mg/mL
Submitted references m(6)A RNA modification and its writer/reader VIRMA/YTHDF3 in testicular germ cell tumors: a role in seminoma phenotype maintenance.
Lobo J, Costa AL, Cantante M, Guimarães R, Lopes P, Antunes L, Braga I, Oliveira J, Pelizzola M, Henrique R, Jerónimo C
Journal of translational medicine 2019 Mar 12;17(1):79
Journal of translational medicine 2019 Mar 12;17(1):79
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of KIAA1429 in human cell line U-2 OS shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a KIAA1429 Polyclonal Antibody (Product # PA5-56772).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of KIAA1429 in human cell line U-2 OS using a KIAA1429 Polyclonal Antibody (Product # PA5-56772) shows localization to nucleoplasm, nuclear bodies and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of KIAA1429 in human breast tissue shows moderate nuclear positivity in glandular cells. Samples were probed using a KIAA1429 Polyclonal Antibody (Product # PA5-56772).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Immunostaining for YTHDF3 ( a , b ), VIRMA ( c , d ); and m 6 A ( e , f ) in testicular germ cell tumors. a Strong YTHDF3 cytoplasmic immunoexpression in embryonal carcinoma; b Weak/moderate YTHDF3 cytoplasmic immunoexpression in postpubertal-type teratoma; c strong VIRMA nuclear immunoexpression in seminoma; d Weak/moderate VIRMA nuclear immunoexpression in Seminoma; e Strong m 6 A nuclear immunostaining in seminoma; f Weak/moderate m 6 A nuclear immunostaining in postpubertal-type yolk sac tumor. Note the granularity of staining, particularly in c , d and e
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Additional file 6: Figure S5. Immunohistochemistry findings in metastatic tumor samples. Comparison between immunostaining intensity of VIRMA (a), YTHDF3 (b) and m 6 A (c) between primary tumor samples and matched metastatic samples; immunostaining intensity of VIRMA (d), YTHDF3 (e) and m 6 A (f) among different tumor subtypes; comparison between immunostaining intensity of VIRMA (g), YTHDF3 (h) and m 6 A (i) in primary and metastatic Teratoma samples. Abbreviations: SE: Seminoma; EC: embryonal carcinoma; YST: pospubertal-type yolk sac tumor; CH: choriocarcinoma; Met: metastases; TE: postpubertal-type Teratoma.
