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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NBP2-32091 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TTLL5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references CSAP Acts as a Regulator of TTLL-Mediated Microtubule Glutamylation.
Bompard G, van Dijk J, Cau J, Lannay Y, Marcellin G, Lawera A, van der Laan S, Rogowski K
Cell reports 2018 Dec 4;25(10):2866-2877.e5
Cell reports 2018 Dec 4;25(10):2866-2877.e5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TTLL5 Antibody [NBP2-32091] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TTLL5 antibody NBP2-32091 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TTLL5 Antibody [NBP2-32091] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TTLL5 antibody NBP2-32091 used for IP at 6 ug per reaction. For blotting immunoprecipitated TTLL5, NBP2-32091 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
