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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
- Other assay [2]
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- Product number
- PA5-58028 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Enkurin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: PAVPLKTDHP VMGIQSGKNF INTNAADIIM GVAKKPKPIY VDKRTGDKHD LEPSGLVPKY INKKDYGVTP EYICKRNEE
- Concentration
- 0.2 mg/mL
Submitted references The flagellar protein Enkurin is required for mouse sperm motility and for transport through the female reproductive tract.
Jungnickel MK, Sutton KA, Baker MA, Cohen MG, Sanderson MJ, Florman HM
Biology of reproduction 2018 Oct 1;99(4):789-797
Biology of reproduction 2018 Oct 1;99(4):789-797
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Enkurin in control (vector only transfected HEK293T lysate) and ENKUR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Enkurin Polyclonal Antibody (Product # PA5-58028).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Enkurin in human bronchus using Enkurin Polyclonal Antibody (Product # PA5-58028).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Enkurin in human kidney using Enkurin Polyclonal Antibody (Product # PA5-58028).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Enkurin in human fallopian tube and colon tissues using Enkurin Polyclonal Antibody (Product # PA5-58028). Corresponding ENKUR RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Enkurin in human bronchus, colon, fallopian tube and kidney using Enkurin Polyclonal Antibody (Product # PA5-58028) (A) shows similar protein distribution across tissues to an independent Enkurin Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Enkurin in human fallopian tube using Enkurin Polyclonal Antibody (Product # PA5-58028) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Enkurin in human colon using Enkurin Polyclonal Antibody (Product # PA5-58028) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
