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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- F47892 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PDX1 Antibody
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1-30 from the human protein was used as the immunogen for this PDX1 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 80 µl, 400 µl
- Storage
- Aliquot the PDX1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of PDX1 antibody and NCI-H460 lysate
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot analysis of PDX1 antibody and 293 cell lysate either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (2).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with PDX1 antibody. Alexa Fluor 488 conjugated secondary (green) was used. Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the PDX1 immunosignal to the nucleus.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC analysis of FFPE human hepatocarcinoma tissue stained with PDX1 antibody
