Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP11898PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11898PU-N, RRID:AB_1770393
- Product name
- anti PDHX / PDX1
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T11 of human PDX1.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of anti-PDX1 (AP11898PU-N) in NCI-H460 cell line lysates (35 µg/lane). PDX1-T11(arrow) was detected using the purified Pab.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of PDX1 (arrow) using PDX1 Antibody (AP11898PU-N). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PDX1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Phospho-PDX1-T11.ctrl antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
