H00005880-M08

antibody from Abnova Corporation

Targeting: RAC2 EN-7

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005880-M08

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005880-M08, RRID:AB_10720051

Product name

RAC2 monoclonal antibody (M08), clone 3B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant RAC2.

Antigen sequence

MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPT
VFDNYSANVMVDSKPVNLGLWDTAGQEDYDRLRPL
SYPQTDVFLICFSLVSPASYENVRAKWFPEVRHHC
PSTPIILVGTKLDLRDDKDTIEKLKEKKLAPITYP
QGLALAKEIDSVKYLECSALTQRGLKTVFDEAIRA
VLCPQPTRQQKRACSLL


Isotype

IgG

Antibody clone number

3B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RAC2 expression in transfected 293T cell line by RAC2 monoclonal antibody (M08), clone 3B8.Lane 1: RAC2 transfected lysate(21.4 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RAC2 is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to RAC2 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol