12926-1-AP

antibody from Invitrogen Antibodies

Targeting: SKAP2 RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12926-1-AP

Provider

Invitrogen Antibodies

Product name

SKAP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPNPSSTSS PYPLPEEIRN LLADVETFVA DILKGENLSK KAKEKRESLI KKIKDVKSIY LQEFQDKGDA EDGEEYDDPF AGPPDTISLA SERYDKDDEA PSDGAQFPPI AAQDLPFVLK AGYLEKRRKD HSFLGFEWQK RWCALSKTVF YYYGSDKDKQ QKGEFAIDGY SVRMNNTLRK DGKKDCCFEI SAPDKRIYQF TAASPKDAEE WVQQLKFVLQ DMESDIIPED YDERGELYDD VDHPLPISNP LTSSQPIDDE IYEELPEEEE DSAPVKVEEQ RKMSQDSVHH TSGDKSTDYA NFYQGLWDCT GAFSDELSFK RGDVIYILSK EYNRYGWWVG EMKGAIGLVP KAYIMEMYDI (1-359 aa encoded by BC036044)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.31 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 12926-1-AP (SKAP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12926-1-AP (SKAP2 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer using 12926-1-AP (SKAP2 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 12926-1-AP ( SKAP2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 12926-1-AP ( SKAP2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 µg Anti-Human SKAP2 (Product # 12926-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.4 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SKAP2 (IP:12926-1-AP, 3ug; Detection:12926-1-AP 1:700) with mouse liver tissue lysate 4000ug.