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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [5]
- Flow cytometry [1]
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- Product number
- MA5-34698 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GSS Recombinant Rabbit Monoclonal Antibody (JB95-33)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: SiHa, mouse colon tissue, rat liver tissue lysate, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, HepG2, LOVO.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JB95-33
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Glutathione Synthetase in Lane 1: SiHa, Lane 2: Mouse colon, Lane 3: Rat liver. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Glutathione Synthetase in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Glutathione Synthetase in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glutathione Synthetase in paraffin-embedded rat epididymis tissue. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glutathione Synthetase in paraffin-embedded mouse colon tissue. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glutathione Synthetase in paraffin-embedded human tonsil tissue. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glutathione Synthetase in paraffin-embedded human kidney tissue. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Glutathione Synthetase in paraffin-embedded human colon cancer tissue. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Glutathione Synthetase in HepG2 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.
