16024-1-AP

antibody from Invitrogen Antibodies

Targeting: LETM1 SLC55A1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16024-1-AP

Provider

Invitrogen Antibodies

Product name

LETM1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EDRLRGQLK QWLDLHLHQE IPTSLLILSR AMYLPDTLSP ADQLKSTLQT LPEIVAKEAQ VKVAEVEGEQ VDNKAKLEAT LQEEAAIQQE HREKELQKRS EVAKDFEPER VVAAPQRPGT EPQPEMPDTV LQSETLKDTA PVLEGLKEEE ITKEEIDILS DACSKLQEQK KSLTKEKEEL ELLKEDVQDY SEDLQEIKKE LSKTGEEKYV EESKASKRLT KRVQQMIGQI DGLISQLEMD QQAGKLAPAN GMPTGENVIS VAELINAMKQ VKHIPESKLT SLAAALDENK DGKVNIDDLV KVIELVDKED VHISTSQVAE IVATLEKEEK VEEKEKAKEK AEKEVAEVKS (391-739 aa encoded by BC021208)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.35 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16024-1-AP (LETM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 16024-1-AP (LETM1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 16024-1-AP) (LETM1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using LETM1 antibody 16024-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 16024-1-AP (LETM1 antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human ovary tumor using 16024-1-AP (LETM1 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-LETM1 (IP:16024-1-AP, 4ug; Detection:16024-1-AP 1:500) with HEK-293 cells lysate 3200ug.