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LearnPA5-113490
antibody from Invitrogen Antibodies
Targeting: VIPAS39
C14orf133, hSPE-39, SPE-39, SPE39, VIPAR, VPS16B
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-113490 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VIPAS39 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 3 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of VIPAS39 in multiple sample types using a VIPAS39 polyclonal antibody (Product # PA5-113490) at a concentration of 12 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:10,000. Sample types that were tested include: Lane 1: HepG2 whole cell lysate, Lane 2: A431 whole cell lysate, Lane 3: 293T whole cell lysate, Lane 4: Mouse kidney tissue, Lane 5: Hela whole cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of VIPAS39 in multiple sample types using a VIPAS39 polyclonal antibody (Product # PA5-113490) at a concentration of 12 µg/ml. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:10,000. Sample types that were tested include: Lane 1: HepG2 whole cell lysate, Lane 2: A431 whole cell lysate, Lane 3: 293T whole cell lysate, Lane 4: Mouse kidney tissue, Lane 5: Hela whole cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemichal analysis of VIPAS39 in Hela cells using a Polyclonal antibody (Product # PA5-113490) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
