12548-1-AP

antibody from Invitrogen Antibodies

Targeting: CPVL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12548-1-AP

Provider

Invitrogen Antibodies

Product name

CPVL Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: YVVTSNMTL RDRDFPWTTT LSMLYIDNPV GTGFSFTDDT HGYAVNEDDV ARDLYSALIQ FFQIFPEYKN NDFYVTGESY AGKYVPAIAH LIHSLNPVRE VKINLNGIAI GDGYSDPESI IGGYAEFLYQ IGLLDEKQKK YFQKQCHECI EHIRKQNWFE AFEILDKLLD GDLTSDPSYF QNVTGCSNYY NFLRCTEPED QLYYVKFLSL PEVRQAIHVG NQTFNDGTIV EKYLREDTVQ SVKPWLTEIM NNYKVLIYNG QLDIIVAAAL TERSLMGMDW KGSQEYKKAE KKVWKIFKSD SEVAGYIRQV GDFHQVIIRG GGHILPYDQP LRAFDMINRF IYGKGWDPYV G (127-476 aa encoded by BC016838)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human kidney tissue were subjected to SDS PAGE followed by western blot with 12548-1-AP (CPVL antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 12548-1-AP (CPVL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 12548-1-AP (CPVL Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver tissue slide using 12548-1-AP (CPVL Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer using 12548-1-AP (CPVL antibody) at dilution of 1:200 (under 40x lens).