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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- TA319576 - Provider product page
- Provider
- OriGene
- Product name
- Mouse monoclonal anti-CENP-E antibody
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal anti-CENP-E antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CENPE
- Antibody clone number
- 1H12
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Anti-CENPE antibody was used to detect CENPE protein, visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A). Interphase cells show no discrete staining (bottom left, panel B). HeLa cells were fixed in paraformaldehyde and stained using this primary antibody. AlexaFluor 555 TM conjugated anti-Mouse antibody (red) was used for detection. DNA was stained using bis-benzimide (DAPI) (green).
- Validation comment
- IF
