MAB1924

antibody from Abnova Corporation

Targeting: CENPE KIF10, PPP1R61

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1924

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB1924, RRID:AB_1672434

Product name

CENPE monoclonal antibody, clone 1H12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against full length recombinant.

Isotype

IgG

Antibody clone number

1H12

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.

Chan GK, Schaar BT, Yen TJ
The Journal of cell biology 1998 Oct 5;143(1):49-63

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CENP-E is a putative kinetochore motor that accumulates just before mitosis.

Yen TJ, Li G, Schaar BT, Szilak I, Cleveland DW
Nature 1992 Oct 8;359(6395):536-9

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CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase.

Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, Cleveland DW
The EMBO journal 1991 May;10(5):1245-54

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence microscopy of CENPE monoclonal antibody, clone 1H12 (Cat # MAB1924) was used to detect CENPE protein visible as discrete nuclear dots on prometaphase and metaphase cells that relocate to the spindle midzone at anaphase (panel A) .Interphase cells show no discrete staining (bottom left, panel B) .HeLa cells were fixed in paraformaldehyde and stained using this primary antibody.AlexaFluor 555 conjugated anti-Mouse antibody (red) was used for detection.DNA was stained using bis-benzimide (DAPI) (green) .Personal Communication, Tim Yen, Fox Chase Cancer Center, Philadelphia, PA.

Validation comment

Immunofluorescence