A15778

antibody from Invitrogen Antibodies

Targeting: CD300A CMRF-35-H9, CMRF35H, IGSF12, IRC1, IRC2, Irp60

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A15778

Provider

Invitrogen Antibodies

Product name

CD300a Monoclonal Antibody (MEM-260), PE

Antibody type

Monoclonal

Antigen

Other

Description

This antibody reacts with an extracellular epitope of CD300a, a 60 kDa leukocyte transmembrane glycoprotein expressed on human granulocytes, monocytes, neutrophils, NK cells, mast cells and dendritic cells, 25% of circulating T cells and 15% of circulating B cells.

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

MEM-260

Vial size

100 Tests

Storage

4° C, store in dark, DO NOT FREEZE!

References

Submitted references

TGF-β1 potentiates Vγ9Vδ2 T cell adoptive immunotherapy of cancer.

Beatson RE, Parente-Pereira AC, Halim L, Cozzetto D, Hull C, Whilding LM, Martinez O, Taylor CA, Obajdin J, Luu Hoang KN, Draper B, Iqbal A, Hardiman T, Zabinski T, Man F, de Rosales RTM, Xie J, Aswad F, Achkova D, Joseph CR, Ciprut S, Adami A, Roider HG, Hess-Stumpp H, Győrffy B, Quist J, Grigoriadis A, Sommer A, Tutt ANJ, Davies DM, Maher J
Cell reports. Medicine 2021 Dec 21;2(12):100473

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 Functional investigation of differentially expressed genes in gammadelta[2] versus gammadelta[T2] cells (A) IL-9 was measured in supernatants harvested on day 15 from gammadelta[2] and gammadelta[T2] cells (values for individual donors are linked by a line; unpaired 2-tailed Student's t test). (B) IL-9 receptor expression was quantified on gammadelta[2] and gammadelta[T2] cells on day 15 using flow cytometry (unpaired 2-tailed Student's t test). (C) PTGER2 protein expression in gammadelta[2] and gammadelta[T2] cells (n = 3 independent donors) was determined by western blotting under reducing conditions. Quantification of band pixel density was performed using ImageJ. Data were normalized for each gammadelta[T2] cell sample with respect to gammadelta[2] cells expanded from the same donor. (D) CD300a was quantified on day 15 from gammadelta[2] and gammadelta[T2] cells using flow cytometry (unpaired Student's t test). (E) The indicated ffLuc-expressing tumor cell lines were co-cultivated with gammadelta[2] and gammadelta[2-ITGAE] cells for 24 h at 1:1 E:T ratio. Tumor cell viability is shown in comparison with tumor cells that had been cultured alone (means +- SDs; 1-way ANOVA). (F) Supernatants were collected from co-cultures described in (E) after 72 h and analyzed for IFN-gamma (means +- SDs; unpaired Student's t test). (G) Effect of exogenous PGE 2 on cytotoxicity (means +- SEMs, n = 10 from 5 independent donors) by gammadelta[2] and gammadelta[T2] cells when co-cul

Conjugate

Yellow dye