PAB20553

antibody from Abnova Corporation

Targeting: MTX1 MTX, MTXN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20553

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20553, RRID:AB_10987406

Product name

MTX1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MTX1.

Antigen sequence

WIDTKNYVEVTRKWYAEAMPFPLNFFLPGRMQRQY
MERLQLLTGEHRPEDEEELEKELYREARECLTLLS
QRLGSQKFFFGDAPASLDAFVFSYLALLLQAKLPS
G


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with MTX1 polyclonal antibody (Cat # PAB20553) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with MTX1 polyclonal antibody (Cat # PAB20553) at 1-4 ug/mL dilution shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach with MTX1 polyclonal antibody (Cat # PAB20553) shows strong cytoplasmic positivity in glandular cells at 1:10-1:20 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)