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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- AF1734 - Provider product page
- Provider
- R&D Systems
- Product name
- Human RECK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human RECK in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O95980- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RECK controls breast cancer metastasis by modulating a convergent, STAT3-dependent neoangiogenic switch.
MT1-MMP and RECK are involved in human CD34+ progenitor cell retention, egress, and mobilization.
miR-21 plays a pivotal role in gastric cancer pathogenesis and progression.
Walsh LA, Roy DM, Reyngold M, Giri D, Snyder A, Turcan S, Badwe CR, Lyman J, Bromberg J, King TA, Chan TA
Oncogene 2015 Apr 23;34(17):2189-203
Oncogene 2015 Apr 23;34(17):2189-203
MT1-MMP and RECK are involved in human CD34+ progenitor cell retention, egress, and mobilization.
Vagima Y, Avigdor A, Goichberg P, Shivtiel S, Tesio M, Kalinkovich A, Golan K, Dar A, Kollet O, Petit I, Perl O, Rosenthal E, Resnick I, Hardan I, Gellman YN, Naor D, Nagler A, Lapidot T
The Journal of clinical investigation 2009 Mar;119(3):492-503
The Journal of clinical investigation 2009 Mar;119(3):492-503
miR-21 plays a pivotal role in gastric cancer pathogenesis and progression.
Zhang Z, Li Z, Gao C, Chen P, Chen J, Liu W, Xiao S, Lu H
Laboratory investigation; a journal of technical methods and pathology 2008 Dec;88(12):1358-66
Laboratory investigation; a journal of technical methods and pathology 2008 Dec;88(12):1358-66
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human RECK by Western Blot. Western blot shows lysates of human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human RECK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1734) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RECK at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
